ENGINEERING HIGHLY EFFICIENT BACKSPLICING AND TRANSLATION OF SYNTHETIC CIRCRNAS

Circular RNAs (circRNAs) are highly stable RNA molecules that are attractive templates for expression of therapeutic proteins and non-coding RNAs.In eukaryotes, circRNAs are primarily generated by the spliceosome through backsplicing.Here, we interrogate different molecular elements including intron type and length, Alu repeats, internal ribosome e